Prerequisites: graduate standing; and consent of instructor

Description: Introduces the methods used to culture human embryonic stem cells (hESC) in vitro. Provides hands-on experience in plating, passaging, culturing, differentiating, and freezing of hESC. Additional topics include staining colonies of hESC for pluripotency markers (including alkaline phosphatase); labeling colonies using immunohistochemistry; and performing chromosome squashes for evaluation of aneuploidy.

Credit: Graded Satisfactory (S) or No Credit (NC).