Units: 5
Hours: Lecture, 1 hour; discussion, 1 hour; laboratory, two 4.5-hour laboratories
Catalog page 117
Prerequisites: BCH 102; BCH 110A, BCH 110B, BCH 110C all with grades of “C+” or better (BCH 110C may be taken concurrently); consent of instructor
Description: Purification, quantitation, and analysis of DNA, RNA, protein, and lipid. Molecular techniques include DNA cloning, in situ hybridization, restriction mapping, PCR, and DNA sequencing. Biochemical techniques include in vitro transcription and translation, immunochemistry, phase extraction, affinity chromatography, and gel shift assays.