OFRG University of California, Riverside

Oligonucleotide Fingerprinting of Ribosomal RNA Genes (OFRG)
and Microorganism Classification

Brief Introduction

The oligonucleotide fingerprinting of ribosomal RNA genes(OFRG) is a method that permits the identification of arrayed ribosomal RNA genes (rDNA) through a series of hybridizaition experiments using small DNA probes. It provides a cost effective means to extensively analyze microbial communities and should have application in medicine, biotechnology and ecosystem studies. This project is funded by NSF DBI. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the author(s) and do not necessarily reflect the views of the National Science Foundation.

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Personnel

James Borneman  Assistant Profesor of Plant Pathology Marek Chrobak  Professor of Computer Science
David Crowley  Associate Professor of Environmental Sciences Gianluca Della Vedova  
Andres Figueroa  Ph.D. Candidate in Computer Science Katechan Jampachaisri  Graduate Student in Statistics
Tao Jiang  Professor of Computer Science Zheng Liu  Graduate student in Computer Science
Jim Press  Distinguished Professor of Statistics Lea Valinsky  

Technical Links

Paper: Oligonucleotide Figerprinting of Ribosomal RNA Genes (OFRG)
CSM 2004 poster A02: Quantitative and qualitative differences in microbial DNA extracted from California soils using three common DNA extraction methods:         Detailed Methods         Results         Poster
Poster: Quantitative and qualitative differences in eubacterial and fungal DNA extracted from California soils using three common DNA extraction methods